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Anti-mTOR (Ser2481) (Polyclonal), ALEXA Fluor 594

Anti-mTOR (Ser2481) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 516
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptidemTOR Ser2481
SpecificityThis phosphorylation site is homologous in the listed cross reactive species at the specified location, mTOR (Ser2481)
ModificationPhosphorylation
Modification siteSer2481
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locations Cell membrane, Lysosome, Mitochondrion, Nucleus, Cytoplasm
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Ser2481
Gene ID2475
Swiss ProtP42345
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis, Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP, Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2), In parallel to protein synthesis, MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins, Moreover, Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor, Stimulates the pyrimidine biosynthesis pathway, This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E), To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A, Under nutrient sufficiency, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1, an allosteric activator of CAD at a later step in synthesis, and delayed regulation, and the inhibitor of translation initiation PDCD4, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, disrupting the interaction with AMPK and preventing activation of ULK1, energy and stress signals, eukaryotic translation initiation factor EIF4B, growth and survival in response to hormones, growth factors, mTORC1 also negatively regulates autophagy through phosphorylation of ULK1, nutrients, phosphorylates ULK1 at 'Ser-758', phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, this function is dependent on the mTORC1 complex, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), Serine/threonine protein kinase which is a central regulator of cellular metabolism
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, RAFT1, RAPT1, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Serine/threonine-protein kinase mTOR, mTOR, FRAP
Also known asmTOR (Ser2481) Polyclonal Antibody
Other nameAnti-mTOR (Ser2481) Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetmTOR (Ser2481)
Short nameAnti-mTOR (Ser2481) (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-Mammalian target on rapamycin (Ser2481) (polyclonal)
Alternative techniquepolyclonals

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