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Anti-PKR (Thr446 + Thr451), ALEXA Fluor 594

Anti-PKR (Thr446 + Thr451), ALEXA Fluor 594 size: 100 microliters 516

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 516
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptidePKR Thr446 + Thr451
SpecificityThis antibody reacts specifically with PKR (Thr446 + Thr451)
ModificationPhosphorylation
Modification siteThr446 + Thr451
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locations Nucleus, Cytoplasm
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human PKR around the phosphorylation site of Thr446/451
Gene ID5610
Swiss ProtP19525
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive speciesHuman (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs, Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6, Also phosphorylates other substrates including p53/TP53, Can act as both a positive and negative regulator of the insulin signaling pathway (ISP), DHX9, Either as an adapter protein and/or via its kinase activity, Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), ILF3, IRF1, IRS1 and the HHV-1 viral protein US11, In addition to serine/threonine-protein kinase activity, Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, PPP2R5A, STAT1, STAT3, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, apoptosis, can regulate various signaling pathways (p38 MAP kinase, cell proliferation and differentiation, facilitating its ubiquitination and proteosomal degradation, hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2), IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms PKR EIF2AK1 PPP1R83
Also known asPKR (Thr446 + Thr451) Antibody
Other nameAnti-PKR (Thr446 + Thr451)
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
Gene targetPKR (Thr446 + Thr451)
Short nameAnti-PKR (Thr446 + Thr451) Fluor 594
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-PKR (Thr446 and Thr451)
Identity 9437
Gene EIF2AK2
Long gene name eukaryotic translation initiation factor 2 alpha kinase 2
Synonyms gene PRKR
Synonyms gene name interferon-inducible double stranded RNA dependent , protein kinase
Synonyms name protein phosphatase 1, regulatory subunit 83
Locus 2p22, 2
Discovery year 1992-12-04
GenBank acession BC057805
Entrez gene record 5610
Pubmed identfication 1351683
RefSeq identity NM_002759
Classification Protein phosphatase 1 regulatory subunits
Havana BLAST/BLAT OTTHUMG00000100962

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