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Anti-RAG2 (Polyclonal), ALEXA Fluor 594

Anti-RAG2 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideRAG2
SpecificityThis antibody reacts specifically with RAG2
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human RAG2
Gene ID5897
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information 5'-phosphorylated ends, DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, Histone H3 monoubiquitination is required for the joining step of V(D)J recombination, In addition to its endonuclease activity, In the RAG complex, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment, RAG2 is not a catalytic component but is required for all known catalytic activities, The RAG complex also plays a role in pre-B cell allelic exclusion, The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps, The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes, and J (joining) gene segments, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, in some cases D (diversity), preventing accessibility to the RAG complex and recombination of the second allele, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, Catalytic component of the RAG complex
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Also known asRAG2 Polyclonal Antibody
Other nameAnti-RAG2 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetRAG2
Short nameAnti-RAG2 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-recombination activating gene 2 (polyclonal)
Alternative techniquepolyclonals
Alternative to gene target RAG-2, RAG2 and IDBG-40186 and ENSG00000175097 and 5897, RAG2 and IDBG-636766 and ENSBTAG00000031309 and 782387, Rag2 and IDBG-193167 and ENSMUSG00000032864 and 19374, nuclei, phosphatidylinositol-4, this GO :0002326 and B cell lineage commitment and biological process this GO :0002331 and pre-B cell allelic exclusion and biological process this GO :0002358 and B cell homeostatic proliferation and biological process this GO :0002360 and T cell lineage commitment and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005546 and phosphatidylinositol-4, this GO :0003677: DNA binding, this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0005515: protein binding and also this GO :0005546: phosphatidylinositol-4, this GO :0003682: chromatin binding, this GO :0005515: protein binding, this GO :0005546: phosphatidylinositol-4, this GO :0005547: phosphatidylinositol-3, this GO :0008270: zinc ion binding, this GO :0035064: methylated histone binding, this GO :0035091: phosphatidylinositol binding, this GO :0043325: phosphatidylinositol-3, this GO :0080025: phosphatidylinositol-3, 4, 4, 4, 4-bisphosphate binding, 4-bisphosphate binding and also this GO :0080025: phosphatidylinositol-3, 4-bisphosphate binding and molecular function this GO :0046622 and positive regulation of organ growth and biological process this GO :0080025 and phosphatidylinositol-3, 5-bisphosphate binding, 5-bisphosphate binding, 5-bisphosphate binding, 5-bisphosphate binding and also this GO :0005547: phosphatidylinositol-3, 5-bisphosphate binding and molecular function, 5-bisphosphate binding and molecular function this GO :0005547 and phosphatidylinositol-3, 5-trisphosphate binding, 5-trisphosphate binding and also this GO :0008270: zinc ion binding and also this GO :0035064: methylated histone binding and also this GO :0035091: phosphatidylinositol binding and also this GO :0043325: phosphatidylinositol-3, 5-trisphosphate binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006310 and DNA recombination and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0016568 and chromatin modification and biological process this GO :0030183 and B cell differentiation and biological process this GO :0030217 and T cell differentiation and biological process this GO :0033077 and T cell differentiation in thymus and biological process this GO :0033151 and V(D)J recombination and biological process this GO :0035064 and methylated histone binding and molecular function this GO :0035091 and phosphatidylinositol binding and molecular function this GO :0043325 and phosphatidylinositol-3, recombination activating gene 2
Identity 9832
Gene RAG2
Long gene name recombination activating 2
Synonyms gene name recombination activating gene 2
Locus 11p13
Discovery year 1990-08-15
GenBank acession AF080577
Entrez gene record 5897
Pubmed identfication 1283330
RefSeq identity NM_000536
Havana BLAST/BLAT OTTHUMG00000166393
Locus Specific Databases RAG2base: Mutation registry for autosomal recessive RAG2 deficiency LRG_99

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