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Anti-TRIM25(Ser100) (Polyclonal), ALEXA Fluor 594

Anti-TRIM25(Ser100) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 516
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideTRIM25 Ser100
SpecificityThis antibody reacts specifically with TRIM25(Ser100)
ModificationPhosphorylation
Modification siteSer100
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Ser100
Gene ID7706
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Expression of the gene is upregulated in response to estrogen, The TRIM motif includes three zinc binding domains, The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, The protein localizes to the cytoplasm, a B box type 1 and a B box type 2, a RING, and a coiled coil region, and it is thought to mediate estrogen actions in breast cancer as a primary response gene, similar to several other members of the TRIM family, The protein encoded by this gene is a member of the tripartite motif (TRIM) family
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms EFP RNF147
Also known asTRIM25(Ser100) Polyclonal Antibody
Other nameAnti-TRIM25(Ser100) Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetTRIM25(Ser100)
Short nameAnti-TRIM25(Ser100) (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-TRIM25(Ser100) (polyclonal)
Alternative techniquepolyclonals
Identity 12932
Gene TRIM25
Long gene name tripartite motif containing 25
Synonyms gene ZNF147
Synonyms gene name zinc finger protein 147 (estrogen-responsive finger protein) tripartite motif-containing 25
Locus 17q23, 1
Discovery year 1994-03-16
GenBank acession D21205
Entrez gene record 7706
Pubmed identfication 7789997
RefSeq identity NM_005082
Classification Tripartite motif containing Ring finger proteins
Havana BLAST/BLAT OTTHUMG00000178124

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