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ATG4D Antibody, Biotin Conjugated

ATG4D Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, ATG4D Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenATG4D
SpecificityThis is a highly specific antibody against ATG4D
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range425-474/474
Subcellular location Mitochondrion, Cytoplasm
SourceKLH conjugated synthetic peptide derived from human ATG4D
Gene ID number84971
Swiss ProtQ86TL0
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, Cysteine protease ATG4D, Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, Has also an activity of delipidating enzyme for the PE-conjugated forms, ROS, The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis, mitophagy and cell viability, to reveal a C-terminal glycine, which is necessary for autophagy, Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms APG4-D
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetATG4D Conjugated
Short name Conjugated, ATG4D Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, ATG4D (Antibody to)
Alternative techniqueantibodies
Identity 20789
Gene ATG4D
Long gene name autophagy related 4D cysteine peptidase
Synonyms gene AUTL4 APG4D
Synonyms gene name AUT-like 4, cerevisiae) APG4 autophagy 4 homolog D (S, cerevisiae) ATG4 autophagy related 4 homolog D (S, cerevisiae) autophagy related 4D, cysteine endopeptidase (S, cysteine peptidase
Locus 19p13, 2
Discovery year 2003-04-11
GenBank acession AJ312332
Entrez gene record 84971
Pubmed identfication 12446702
RefSeq identity NM_032885
Classification Autophagy related
Havana BLAST/BLAT OTTHUMG00000180582

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