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ATG4D Antibody, FITC Conjugated

ATG4D Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, ATG4D Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenATG4D
SpecificityThis is a highly specific antibody against ATG4D
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range425-474/474
Subcellular location Mitochondrion, Cytoplasm
SourceKLH conjugated synthetic peptide derived from human ATG4D
Gene ID number84971
Swiss ProtQ86TL0
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, Cysteine protease ATG4D, Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, Has also an activity of delipidating enzyme for the PE-conjugated forms, ROS, The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis, mitophagy and cell viability, to reveal a C-terminal glycine, which is necessary for autophagy, Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms APG4-D
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetATG4D Conjugated
Short name FITC Conjugated, ATG4D Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ATG4D (Antibody to)
Alternative techniqueantibodies
Identity 20789
Gene ATG4D
Long gene name autophagy related 4D cysteine peptidase
Synonyms gene AUTL4 APG4D
Synonyms gene name AUT-like 4, cerevisiae) APG4 autophagy 4 homolog D (S, cerevisiae) ATG4 autophagy related 4 homolog D (S, cerevisiae) autophagy related 4D, cysteine endopeptidase (S, cysteine peptidase
Locus 19p13, 2
Discovery year 2003-04-11
GenBank acession AJ312332
Entrez gene record 84971
Pubmed identfication 12446702
RefSeq identity NM_032885
Classification Autophagy related
Havana BLAST/BLAT OTTHUMG00000180582

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