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Beta Actin Antibody Loading Control

Beta Actin Antibody Loading Control size: 0.1mg 320

Supplier NJS POLY
Price 320
Size 0.1mg
CategoryAntibody
Concentration05% sodium azide, 1 mg/ml BSA (US sourced) and 0, 5 mg/ml in 1X PBS with 0
FormPurified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenBeta Actin Loading Control
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Chicken, Dog , Due to limited knowledge and inability to test the antibody against all known species, Monkey, Mouse (Mus musculus), Rabbit (Oryctolagus cuniculus), Rat, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applicationsWB
Recommended dilutionsWestern blot: 1-3ug/ml
Notes beta Actin antibody titration may be required for optimal results, Provided assay concentrations are suggestions only
Added buffer pH 7, 1X Phosphate Buffered Saline (PBS), 4
Intented useThis beta Actin antibody loading controlis to be used only for research purposes and not for diagnostics
Gene ID #60
PurityPurified by protein A Affinity Chromatography
Description Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins, An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, Beta actin is commonly used as a loading control protein in western blot, There are three main isoforms of actin in vertebrates: alpha, beta and gamma, integrity and motility, typically transfected vs mock or non transfected cell lysate, Actins proteins are highly conserved and involved in cellular structure
ImmunogenA recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody
StorageAliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetBeta Actin Loading
Short nameBeta Actin Antibody Loading Control
Technique antibodies against human proteins, antibodies for, controls, Antibody
Alternative nameb Actin (Antibody to) Loading reference
Alternative techniqueantibodies

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