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BRD4 Antibody

BRD4 Antibody size: 0.08 ml 199

Supplier NJS POLY
Price 199
Size 0.08 ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration pH 7, with 0, 09% sodium azide, 4, In 1X PBS
FormAntigen affinity purified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenBRD4
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applications ELISA, WB
Recommended dilutionsWestern blot: 1:1000
NotesTitration of the BRD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity
Intented useThis BRD4 antibodyis to be used only for research purposes and not for diagnostics
Uniprot #O60885
PurityAntigen affinity
Description According to a report, Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, During interphase, In addition to acetylated histones, Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, Isoform B: Acts as a chromatin insulator in the DNA damage response pathway, Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II, Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure, [UniProt], also recognizes and binds acetylated RELA, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II, insulating the region from DNA damage response by limiting spreading of histone H2AFX/H2A, leading to chromatin structure remodeling, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, recruited to p53/TP53 specific target promoters, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II, these data however need additional evidences in vivo (PubMed:22509028), Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation, x phosphorylation
ImmunogenA portion of amino acids 1160-1188 from the human protein was used as the immunogen for this BRD4 antibody
Storage Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the BRD4 antibody and store frozen at -20 deg
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetBRD4
Short nameBRD4 Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameBRD4 (Antibody to)
Alternative techniqueantibodies
Identity 13575
Gene BRD4
Long gene name bromodomain containing 4
Synonyms gene name bromodomain-containing 4
Synonyms HUNKI MCAP CAP HUNK1
Synonyms name chromosome-associated protein
Locus 19p13, 12
Discovery year 2000-09-26
GenBank acession Y12059
Entrez gene record 23476
Pubmed identfication 10938129
RefSeq identity NM_058243
Havana BLAST/BLAT OTTHUMG00000183252

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