| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | CD47 |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | IHC-P, WB |
| Recommended dilutions | 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes | Titration of the CD47 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use | This CD47 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | Q08722 |
| Purity | Antigen affinity |
| Description | CD47 has broad tissue distribution, It is also a receptor for the C-terminal cell binding domain of thrombospondin, The protein belongs to the immunoglobulin superfamily and is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix, also known as IAP or MER6, and is reduced in expression on Rh erythrocytes, and it may play a role in membrane transport and signal transduction, is a transmembrane protein that in humans is encoded by the CD47 gene, CD47 |
| Immunogen | An amino acid sequence from the C-terminus of human Cluster of Differentiation 47 (EPLNAFKESKGMMNDE) was used as the immunogen for this CD47 antibody (100% rat homology) |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CD47 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | CD47 |
| Short name | CD47 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | CD47 molecule (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | CD47 and IDBG-48744 and ENSG00000196776 and 961, CD47 and IDBG-631689 and ENSBTAG00000003585 and 282661, Cd47 and IDBG-165137 and ENSMUSG00000055447 and 16423, IAP and MER6 and OA3, Plasma membranes, this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007229 and integrin-mediated signaling pathway and biological process this GO :0007596 and blood coagulation and biological process this GO :0008228 and opsonization and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009617 and response to bacterium and biological process this GO :0022409 and positive regulation of cell-cell adhesion and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0050766 and positive regulation of pha this GO cytosis and biological process this GO :0050870 and positive regulation of T cell activation and biological process this GO :0050900 and leukocyte migration and biological process this GO :0070053 and thrombospondin receptor activity and molecular function this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0070053: thrombospondin receptor activity, this GO :0070053: thrombospondin receptor activity, thrombospondin receptor activity, CD47 molecule |
| Identity | 1682 |
| Gene | CD47 |
| Long gene name | CD47 molecule |
| Synonyms gene | MER6 |
| Synonyms gene name | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) |
| Synonyms | IAP OA3 |
| Synonyms name | antigen identified by monoclonal antibody 1D8 antigenic surface determinant protein OA3 integrin associated protein Rh-related antigen leukocyte surface antigen CD47 CD47 glycoprotein |
| Locus | 3q13, 12 |
| Discovery year | 1994-12-12 |
| Entrez gene record | 961 |
| Pubmed identfication | 8294396 2277087 |
| RefSeq identity | NM_001777 |
| Classification | Immunoglobulin like domain containing CD molecules |
| Havana BLAST/BLAT | OTTHUMG00000044216 |
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