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CHK2 (ser516) Antibody, ALEXA FLUOR 488

CHK2 (ser516) Antibody, ALEXA FLUOR 488 size: 100ul 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES
Price 350
Size 100ul
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name ALEXA FLUOR 488 Conjugated, CHK2 (ser516) Polyclonal Antibody
Also known asAnti-CHK2 ser516 PAb ALEXA FLUOR 488
CategoryConjugated Primary Antibodies
Conjugated with 488, ALEXA FLUOR®
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenCHK2 ser516
SpecificityThis is a highly specific antibody against CHK2 ser516
ModificationPhosphorylation
Modification Siteser516
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of ser516
Gene ID Number11200
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Cross-reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the antigen Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, Chk2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases, Indeed, Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block, Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif), The SQ/TQ cluster domain, The amino-terminal domain of Chk2 contains a series of seven serine and threonine residues (Ser19, These are known to be preferred sites for phosphorylation by ATM/ATR kinases, This kinase may have a role in meiosis as well, Thr26, Thr68 and other sites in this region become phosphorylated by ATM/ATR, UV irradiation or hydroxyurea treatment, after DNA damage by ionizing radiation (IR), and may also participate in transduction of the DNA damage and replicational stress signals, preventing the entry into mitosis, seems to have a regulatory function, therefore, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain, Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints
PurificationPurified by Protein A
Storage conditions 50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA
Excitation emission499nm/519nm
Synonyms CDS1 CHK2 HuCds1 PP1425 bA444G7
Properties ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the CHK2 (ser516) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
Gene targetCHK2 (ser516)
Short nameCHK2 (ser516) Antibody
Technique antibodies against human proteins, antibodies for, Antibody
LabelALEXA FLUOR 488
Alternative name ALEXA FLUOR 488, CHK2 (ser516) (Antibody to)
Alternative techniqueantibodies
Identity 16627
Gene CHEK2
Long gene name checkpoint kinase 2
Synonyms gene RAD53
Synonyms gene name CHK2 (checkpoint, S, pombe) , pombe) homolog CHK2 checkpoint homolog (S
Locus 22q12, 1
Discovery year 2001-09-19
GenBank acession AF086904
Entrez gene record 11200
Pubmed identfication 9836640 10097108
RefSeq identity NM_001005735
Havana BLAST/BLAT OTTHUMG00000151023
Locus Specific Databases LRG_302

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