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Cubilin (CUBN) ELISA kit
Cubilin (CUBN) ELISA kit size: 1 plate of 96 wells 822
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BIOMATIK ELISA KITS
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822
Size
1 plate of 96 wells
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| Antigen detectable with this ELISA kit | Cubilin (CUBN) |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 0, 1, 25ng/mL, 3, 6, 12, 125ng/mL, 25ng/mL, 563ng/mL, 5ng/mL, 781-50ng/mL The standard curve concentrations used for the ELISA&rsquo, 781ng/mL, s were 50ng/mL |
| Sensitivity | 34ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application | ELISA |
| Sample types tested for this ELISA kit | Tissue homogenates and other biological fluids |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cubilin (CUBN), The concentration of Cubilin (CUBN) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cubilin (CUBN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cubilin (CUBN), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | Cubilin (CUBN) |
| Short name | Cubilin (CUBN) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | Cubilin (cubilin (intrinsic factor-cobalamin receptor)) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | CUBN and IDBG-58020 and ENSG00000107611 and 8029, CUBN and IDBG-638261 and ENSBTAG00000005072 and 523202, Cubn and IDBG-137553 and ENSMUSG00000026726 and 65969, Plasma membranes, gp280 and IFCR and MGA1, protein homodimerization activity, this GO :0001894 and tissue homeostasis and biological process this GO :0004872 and receptor activity and molecular function this GO :0005215 and transporter activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005765 and lysosomal membrane and cellular component this GO :0005768 and endosome and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005903 and brush border and cellular component this GO :0005905 and coated pit and cellular component this GO :0006766 and vitamin metabolic process and biological process this GO :0006767 and water-soluble vitamin metabolic process and biological process this GO :0006898 and receptor-mediated endocytosis and biological process this GO :0008202 and steroid metabolic process and biological process this GO :0008203 and cholesterol metabolic process and biological process this GO :0009235 and cobalamin metabolic process and biological process this GO :0010008 and endosome membrane and cellular component this GO :0015889 and cobalamin transport and biological process this GO :0016020 and membrane and cellular component this GO :0016324 and apical plasma membrane and cellular component this GO :0030139 and endocytic vesicle and cellular component this GO :0031232 and extrinsic component of external side of plasma membrane and cellular component this GO :0031419 and cobalamin binding and molecular function this GO :0031526 and brush border membrane and cellular component this GO :0042157 and lipoprotein metabolic process and biological process this GO :0042359 and vitamin D metabolic process and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0042953 and lipoprotein transport and biological process this GO :0043202 and lysosomal lumen and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0045177 and apical part of cell and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004872: receptor activity, this GO :0004872: receptor activity and also this GO :0005215: transporter activity and also this GO :0005509: calcium ion binding and also this GO :0005515: protein binding and also this GO :0031419: cobalamin binding and also this GO :0042803: protein homodimerization activity, this GO :0005215: transporter activity, this GO :0005509: calcium ion binding, this GO :0005515: protein binding, this GO :0031419: cobalamin binding, this GO :0042803: protein homodimerization activity, cubilin (intrinsic factor-cobalamin receptor) |
| Identity | 2548 |
| Gene | CUBN |
| Long gene name | cubilin |
| Synonyms gene | MGA1 |
| Synonyms gene name | cubilin (intrinsic factor-cobalamin receptor) |
| Synonyms | IFCR gp280 |
| Synonyms name | intrinsic factor-cobalamin receptor |
| Locus | 10p13 |
| Discovery year | 1998-11-02 |
| GenBank acession | AF034611 |
| Entrez gene record | 8029 |
| Pubmed identfication | 9572993 9478979 |
| RefSeq identity | NM_001081 |
| Havana BLAST/BLAT | OTTHUMG00000017741 |
| Locus Specific Databases | LRG_540 |