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E2F2 Antibody

E2F2 Antibody size: 0.1mg 389

Supplier NJS POLY
Price 389
Size 0.1mg
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration2ml sterile DI water, 5mg/ml if reconstituted with 0
FormAntigen affinity purified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenE2F2
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applicationsWB
Recommended dilutions5-1ug/ml, Western blot: 0
Notes Titration of the E2F2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts
Intented useThis E2F2 antibodyis to be used only for research purposes and not for diagnostics
Uniprot #Q14209
PurityAntigen affinity
Description Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, In contrast, The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses, The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4, The gene is mapped to 1p36 by fluorescence in situ hybridization, also called E2F-2, is a member of the E2F family of transcription factors, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C, E2F transcription factor 2
ImmunogenAn amino acid sequence from the C-terminus of human E2F transcription factor 2 (ISDLFDSYDLGDLLIN) was used as the immunogen for this E2F2 antibody (100% mouse homology)
Storage Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the E2F2 antibody can be stored for up to one month at 4oC, After reconstitution
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetE2F2
Short nameE2F2 Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameE2F transcription factor 2 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target DNA-templated and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0051726 and regulation of cell cycle and biological process this GO :0072332 and intrinsic apoptotic signaling pathway by p53 class mediator and biological process this GO :1990086 and lens fiber cell apoptotic process and biological process, E2F-2, E2F2 and IDBG-645457 and ENSBTAG00000014400 and 617024, E2F2 and IDBG-93950 and ENSG00000007968 and 1870, E2f2 and IDBG-197705 and ENSMUSG00000018983 and 242705, nuclei, this GO :0000278 and mitotic cell cycle and biological process this GO :0001047 and core promoter binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0006355 and regulation of transcription, this GO :0001047: core promoter binding, this GO :0001047: core promoter binding and also this GO :0003677: DNA binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0005515: protein binding and also this GO :0008134: transcription factor binding, this GO :0003677: DNA binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0005515: protein binding, this GO :0008134: transcription factor binding, transcription factor binding, E2F transcription factor 2
Identity 3114
Gene E2F2
Long gene name E2F transcription factor 2
Synonyms E2F-2
Locus 1p36, 12
Discovery year 1994-08-31
GenBank acession L22846
Entrez gene record 1870
Pubmed identfication 8246995 8246996
RefSeq identity NM_004091
Classification E2F transcription factors
Havana BLAST/BLAT OTTHUMG00000003223

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