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GAD65 + GAD67 Antibody, Biotin Conjugated

GAD65 + GAD67 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, GAD65 + GAD67 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenGAD65 + GAD67
SpecificityThis is a highly specific antibody against GAD65 + GAD67
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range370-420/585
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human GAD65
Gene ID number25722571
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes, The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid, This gene may also play a role in the stiff man syndrome, identified as a major autoantigen in insulin-dependent diabetes, This gene encodes one of several forms of glutamic acid decarboxylase
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms GAD65
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetGAD65 + GAD67 Conjugated
Short name Conjugated, GAD65 + GAD67 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, GAD65 and GAD67 (Antibody to)
Alternative techniqueantibodies
Identity 4093
Gene GAD2
Long gene name glutamate decarboxylase 2
Synonyms gene name 65kD) glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 (pancreatic islets and brain
Locus 10p12, 1
Discovery year 1991-11-29
GenBank acession AJ251501
Entrez gene record 2572
Pubmed identfication 2039509
RefSeq identity NM_000818
Havana BLAST/BLAT OTTHUMG00000017836

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