Name : GAPDH Loading Control Antibody
Supplier : NJS POLY
Price :406
SKU : GEN7550742440
| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | GAPDH Loading Control |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | WB |
| Recommended dilutions | 5-1ug/ml, Western blot: 0 |
| Notes | Titration of the GAPDH loading control antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use | This GAPDH loading control antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID # | 2597 |
| Purity | Antigen affinity |
| Description | GAPDH antibody is commonly used as a western blot loading control, GAPDH is mapped to 12p13, The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), 31, Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules |
| Immunogen | Amino acids YSNRVVDLMAHMASKE were used as the immunogen for this GAPDH loading control antibody |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the GAPDH loading control antibody can be stored for up to one month at 4oC, After reconstitution |
| Localization | Nuclear and cytoplasmic |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | GAPDH Loading |
| Short name | GAPDH Loading Control Antibody |
| Technique | antibodies against human proteins, antibodies for, controls, Antibody |
| Alternative name | GAPDH Loading reference (Antibody to) |
| Alternative technique | antibodies |
| Identity | 4141 |
| Gene | GAPDH |
| Long gene name | glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms gene | GAPD |
| Locus | 12p13, 31 |
| Discovery year | 2001-06-22 |
| GenBank acession | AF261085 |
| Entrez gene record | 2597 |
| Pubmed identfication | 3170585 |
| RefSeq identity | NM_002046 |
| Havana BLAST/BLAT | OTTHUMG00000137379 |