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GAPDH Loading Control Antibody

GAPDH Loading Control Antibody size: 0.1mg 406

Supplier NJS POLY
Price 406
Size 0.1mg
CategoryAntibody
Concentration2ml sterile DI water, 5mg/ml if reconstituted with 0
FormAntigen affinity purified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenGAPDH Loading Control
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applicationsWB
Recommended dilutions5-1ug/ml, Western blot: 0
NotesTitration of the GAPDH loading control antibody may be required due to differences in protocols and secondary/substrate sensitivity
Intented useThis GAPDH loading control antibodyis to be used only for research purposes and not for diagnostics
Gene ID #2597
PurityAntigen affinity
Description GAPDH antibody is commonly used as a western blot loading control, GAPDH is mapped to 12p13, The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), 31, Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules
ImmunogenAmino acids YSNRVVDLMAHMASKE were used as the immunogen for this GAPDH loading control antibody
Storage Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the GAPDH loading control antibody can be stored for up to one month at 4oC, After reconstitution
LocalizationNuclear and cytoplasmic
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetGAPDH Loading
Short nameGAPDH Loading Control Antibody
Technique antibodies against human proteins, antibodies for, controls, Antibody
Alternative nameGAPDH Loading reference (Antibody to)
Alternative techniqueantibodies
Identity 4141
Gene GAPDH
Long gene name glyceraldehyde-3-phosphate dehydrogenase
Synonyms gene GAPD
Locus 12p13, 31
Discovery year 2001-06-22
GenBank acession AF261085
Entrez gene record 2597
Pubmed identfication 3170585
RefSeq identity NM_002046
Havana BLAST/BLAT OTTHUMG00000137379

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