Name : His Antibody
Supplier : NJS POLY
Price :199
SKU : GEN9403668115
| Category | Antibody |
| Concentration | 09% sodium azide, 4 with 0, In 1X PBS pH 7 |
| Form | Purified |
| Conjugation | histidine |
| Clone | Polyclonal antibody |
| Recognised antigen | His |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur |
| Tested applications | ELISA, WB |
| Recommended dilutions | Western blot: 1:1000 |
| Notes | Titration of the His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use | This His Tag antibodyis to be used only for research purposes and not for diagnostics |
| Purity | Purified |
| Description | 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin, NSJBio's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins, The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application, Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems |
| Immunogen | Poly-His peptide was used as the immungen for the His Tag antibody |
| Storage | Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the His Tag antibody and store frozen at -20 deg |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | His |
| Short name | His Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Label | His |
| Alternative name | histidine (Antibody to) |
| Alternative technique | antibodies |
| Identity | 4918 |
| Gene | HIS |
| Long gene name | histidine ammonia-lyase |
| Synonyms | HAL |
| Locus | 12q23, 1 |
| Discovery year | 1988-08-31 |
| Entrez gene record | 3034 |
| Havana BLAST/BLAT | OTTHUMG00000170354 |