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Insulin Receptor Beta Antibody, FITC Conjugated

Insulin Receptor Beta Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, Insulin Receptor Beta Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenInsulin Receptor Beta
SpecificityThis is a highly specific antibody against Insulin Receptor Beta
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range1020-1070/1382
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human IR beta
Gene ID number3643
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background IRS1, IRS2 and Shc, RTKs are essential components of signal transduction pathways that affect cell proliferation, RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, Receptor activation occurs through ligand binding, The beta subunit (95 kDa) possesses a single transmembrane domain, The insulin receptor exhibits receptor tyrosine kinase (RTK) activity, The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, These three phosphorylated proteins then interact with SH2 domain containing signaling proteins, Three adapter proteins, become phosphorylated on tyrosine residues following insulin receptor activation, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates, differentiation, fibroblast growth factor and vascular endothelial growth factor, migration and metabolism, whereas the alpha subunit (135 kDa) is completely extracellular, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion, which then undergoes an autophosphorylation that increases its tyrosine kinase activity, Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms CD220, CD220 antigen, HHF 5, HHF5, HIR B, INSR, INSR, INSR_HUMAN, IR, Insulin receptor, Insulin receptor subunit beta, CD 220
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetInsulin Receptor Beta Conjugated
Short name FITC Conjugated, Insulin Receptor Beta Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Insulin Receptor b (Antibody to)
Alternative techniqueantibodies

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