| Long name | IRAK4 Primary Polyclonal Antibody |
| Also known as | IRAK4 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | IRAK4 |
| Specificity | This is a highly specific antibody against IRAK4 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 20-55/460 |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human IRAK4 |
| Gene ID number | 51135 |
| Swiss Prot | Q9NWZ3 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Alternatively, In turn, Involved in Toll-like receptor (TLR) and IL-1R signaling pathways, Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1, Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections, Phosphorylates initially IRAK1, Then, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1, Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | IRAK-4, IRAK4, Interleukin-1 receptor-associated kinase 4, NY-REN-64, REN64, Renal carcinoma antigen NY-REN-64, IPD1 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | IRAK4 |
| Short name | IRAK4 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | interleukin-1 receptor-associated kinase 4 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | Extracellular, IRAK4 and IDBG-28022 and ENSG00000198001 and 51135, IRAK4 and IDBG-633310 and ENSBTAG00000021105 and 533692, Irak4 and IDBG-177236 and ENSMUSG00000059883 and 266632, this GO :0000287 and magnesium ion binding and molecular function this GO :0001816 and cytokine production and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005149 and interleukin-1 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007254 and JNK cascade and biological process this GO :0010008 and endosome membrane and cellular component this GO :0016772 and transferase activity, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0004672: protein kinase activity and also this GO :0004674: protein serine/threonine kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005149: interleukin-1 receptor binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004672: protein kinase activity, this GO :0004674: protein serine/threonine kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005149: interleukin-1 receptor binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034146 and toll-like receptor 5 signaling pathway and biological process this GO :0034162 and toll-like receptor 9 signaling pathway and biological process this GO :0034166 and toll-like receptor 10 signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0045087 and innate immune response and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process, interleukin-1 receptor-associated kinase 4 |
| Identity | 17967 |
| Gene | IRAK4 |
| Long gene name | interleukin 1 receptor associated kinase 4 |
| Synonyms | NY-REN-64 |
| Locus | 12q12 |
| Discovery year | 2002-09-27 |
| GenBank acession | AF155118 |
| Entrez gene record | 51135 |
| Pubmed identfication | 3772297 10508479 |
| Havana BLAST/BLAT | OTTHUMG00000169425 |
| Locus Specific Databases | IRAK4base: Mutation registry for IRAK4 deficiency LRG_75 |
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