Name : MARCH9 Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN2173958144
| Long name | FITC Conjugated, MARCH9 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | 39873 |
| Specificity | This is a highly specific antibody against 39873 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human MARCH9 |
| Gene ID number | 92979 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Expressed ubiquitously, MARCH9 (membrane-associated ring finger (C3HC4) 9), MARCH9 exists as a homodimer and functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and is thought to promote the degradation of target proteins, Multiple isoforms of MARCH9 exist due to alternative splicing events, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), also known as RNF179, is a 346 amino acid multi-pass membrane protein that localizes to the golgi apparatus and contains one RING-CH-type zinc finger, such as CD4 and MHC-I, the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | RNF179 FLJ36578 |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | MARCH9 Conjugated |
| Short name | FITC Conjugated, MARCH9 Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, MARCH9 (Antibody to) |
| Alternative technique | antibodies |
| Identity | 25139 |
| Gene | MARCH9 |
| Long gene name | membrane associated ring-CH-type finger 9 |
| Synonyms gene name | membrane-associated ring finger (C3HC4) 9 |
| Locus | 12q14, 1 |
| Discovery year | 2005-01-26 |
| GenBank acession | BC009489 |
| Entrez gene record | 92979 |
| Pubmed identfication | 14722266 |
| RefSeq identity | NM_138396 |
| Classification | Membrane associated ring-CH-type fingers Ring finger proteins |
| Havana BLAST/BLAT | OTTHUMG00000170456 |