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MDM2(Ser186) Antibody, Biotin Conjugated

MDM2(Ser186) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, MDM2(Ser186) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMDM2 Ser186
SpecificityThis is a highly specific antibody against MDM2 Ser186
ModificationPhosphorylation
Modification siteSer186
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range165-210/491
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human MDM2 around the phosphorylation site of Ser186
Gene ID number4193
Swiss ProtQ00987
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Also acts as a ubiquitin ligase E3 toward itself and ARRB1, Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways, Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53, Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation, Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain, Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus, Permits the nuclear export of p53/TP53, Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein, Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation, leading to its degradation by the proteasome, E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms HDM2 MGC5370
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetMDM2(Ser186) Conjugated
Short name Conjugated, MDM2(Ser186) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, MDM2(Ser186) (Antibody to)
Alternative techniqueantibodies
Identity 6973
Gene MDM2
Long gene name MDM2 proto-oncogene
Synonyms gene name E3 ubiquitin protein ligase , human homolog of, mouse double minute 2, p53 binding protein (mouse) Mdm2 p53 binding protein homolog (mouse) MDM2 proto-oncogene, p53-binding protein Mdm2, transformed 3T3 cell double minute 2
Locus 12q15
Discovery year 1993-12-10
Entrez gene record 4193
Pubmed identfication 1614537 16905769
RefSeq identity NM_006880
Classification Zinc fingers RANBP2-type
Havana BLAST/BLAT OTTHUMG00000142827

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