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MOUSE Anti-HUMAN HLA B27:FITC Antibody

MOUSE Anti-HUMAN HLA B27:FITC Antibody size: 100 Tests 702

Supplier MBS MONO
Price 702
Size 100 Tests
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Also known asHLA B27
Other names B, B, B alpha chain, B-27 alpha chain, B-27 alpha chain, HLA class I histocompatibility antigen, HLA class I histocompatibility antigen, MHC Class I HLA heavy chain, MHC HLA-B cell surface glycoprotein, MHC HLA-B transmembrane glycoprotein, MHC class I antigen B*27, MHC class I antigen GN00104, MHC class I antigen HLA-B heavy chain, MHC class I antigen SHCHA, class I, class I, leukocyte antigen class I-B, lymphocyte antigen, major histocompatibility complex, major histocompatibility complex, HLA class I histocompatibility antigen
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameHLA B27
Gene name synonimsN/A
Other gene names AS, HLA-B, HLAB, HLAB, SPDA1, HLA-B
ClonalityMonoclonal
Immunoglobulin isotypeIgG2a
CloneHLA-ABC-m3
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens)
Specificity and cross-reactivity Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9, M<, Non B7 Negative, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, This particular antibody might cross react with speacies outside of the listed ones, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin, ), ) than for HLA B7 (9, -1<, -1<, /sup>, /sup>, /sup>, /sup>, 5 x 10<, 7 x 10<, 7<, 8<, HLA B27 HLA-ABC-m3 is a cytotoxic antibody which detects HLA-B27 alloantigen, M<, sup>, sup>, sup>, sup>
Purification methodN/A
Form/AppearanceFITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Concentration50 ug/ml
Storage and shipping DO NOT FREEZE, If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Shelf Life: 18 months from date of dispatch, Temperature variations in the range between +1C to +7C are tolerable, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Keep the antibody refrigerated at +4 degrees Celsius
Tested applications:Flow Cytometry (FACS)
Properties FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
ConjugationAnti-FITC Antibody
Gene targetHLA B27:
Short nameMOUSE Anti-HUMAN HLA B27:FITC Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, Antibody
LabelFITC
Alternative name sapiens Human leukocyte antigen B27:fluorecein (Antibody to), MOUSE antibody to-H
Alternative techniqueantibodies

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