| Long name | 2) Monoclonal Antibody, 3, MSH2 (1184CT1 |
| Also known as | 2) Monoclonal Antibody, 3, MSH2 (1184CT1 |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | MSH2 |
| Specificity | This is a highly specific antibody against MSH2 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Monoclonal |
| Clone number | 1184CT1, 2, 3 |
| Source | Purified His-tagged MSH2 protein was used to produced this monoclonal antibody |
| Gene ID number | 4436 |
| Swiss Prot | P43246 |
| Tested Applications | WB |
| Recommended dilutions | WB(1:2000) |
| Cross reactivity | Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | ATP binding and hydrolysis play a pivotal role in mismatch repair functions, After mismatch binding, Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair, In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis, MutS alpha may also play a role in DNA homologous recombination repair, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA, MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long, The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->, This transition is crucial for mismatch repair, When bound, and resynthesis, excision, heterodimers bend the DNA helix and shields approximately 20 base pairs, including strand discrimination, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone, which is thought to be responsible for directing the downstream MMR events, ATP exchange, Component of the post-replicative DNA mismatch repair system (MMR) |
| Purification method | This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0 |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | COCA1, DNA mismatch repair protein Msh2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2, hMSH2, FCC1 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target | 2) Monoclonal, 3, MSH2 (1184CT1 |
| Short name | 2) Monoclonal Antibody, 3, MSH2 (1184CT1 |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name | coli) (1184CT1, colon cancer, nonpolyposis type 1 (E, 2) monoclonal (Antibody to), 3, mutS homolog 2 |
| Alternative technique | antibodies |
| Alternative to gene target | ADP binding, COCA1 and FCC1 and HNPCC and HNPCC1 and LCFS2, MSH2 and IDBG-50900 and ENSG00000095002 and 4436, MSH2 and IDBG-633627 and ENSBTAG00000002742 and 533115, Msh2 and IDBG-201483 and ENSMUSG00000024151 and 17685, coli), colon cancer, nonpolyposis type 1 (E, nuclei, this GO :0000228 and nuclear chromosome and cellular component this GO :0000287 and magnesium ion binding and molecular function this GO :0000400 and four-way junction DNA binding and molecular function this GO :0000403 and Y-form DNA binding and molecular function this GO :0000404 and heteroduplex DNA loop binding and molecular function this GO :0000406 and double-strand/single-strand DNA junction binding and molecular function this GO :0000710 and meiotic mismatch repair and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0002204 and somatic recombination of immunoglobulin genes involved in immune response and biological process this GO :0003677 and DNA binding and molecular function this GO :0003684 and damaged DNA binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0003697 and single-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0006119 and oxidative phosphorylation and biological process this GO :0006200 and ATP catabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006298 and mismatch repair and biological process this GO :0006301 and postreplication repair and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006311 and meiotic gene conversion and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007281 and germ cell development and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008094 and DNA-dependent ATPase activity and molecular function this GO :0008340 and determination of adult lifespan and biological process this GO :0008584 and male this GO nad development and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0010165 and response to X-ray and biological process this GO :0010224 and response to UV-B and biological process this GO :0016020 and membrane and cellular component this GO :0016446 and somatic hypermutation of immunoglobulin genes and biological process this GO :0016447 and somatic recombination of immunoglobulin gene segments and biological process this GO :0016887 and ATPase activity and molecular function this GO :0019237 and centromeric DNA binding and molecular function this GO :0019724 and B cell mediated immunity and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030183 and B cell differentiation and biological process this GO :0030983 and mismatched DNA binding and molecular function this GO :0031573 and intra-S DNA damage checkpoint and biological process this GO :0032137 and guanine/thymine mispair binding and molecular function this GO :0032139 and dinucleotide insertion or deletion binding and molecular function this GO :0032142 and single guanine insertion binding and molecular function this GO :0032143 and single thymine insertion binding and molecular function this GO :0032181 and dinucleotide repeat insertion binding and molecular function this GO :0032301 and MutSalpha complex and cellular component this GO :0032302 and MutSbeta complex and cellular component this GO :0032357 and oxidized purine DNA binding and molecular function this GO :0032405 and MutLalpha complex binding and molecular function this GO :0042771 and intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0043531 and ADP binding and molecular function this GO :0043570 and maintenance of DNA repeat elements and biological process this GO :0045128 and negative regulation of reciprocal meiotic recombination and biological process this GO :0045190 and isotype switching and biological process this GO :0045910 and negative regulation of DNA recombination and biological process this GO :0051096 and positive regulation of helicase activity and biological process, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0000400: four-way junction DNA binding and also this GO :0000403: Y-form DNA binding and also this GO :0000404: heteroduplex DNA loop binding and also this GO :0000406: double-strand/single-strand DNA junction binding and also this GO :0003677: DNA binding and also this GO :0003684: damaged DNA binding and also this GO :0003690: double-stranded DNA binding and also this GO :0003697: single-stranded DNA binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0008022: protein C-terminus binding and also this GO :0008094: DNA-dependent ATPase activity and also this GO :0016887: ATPase activity and also this GO :0019237: centromeric DNA binding and also this GO :0019899: enzyme binding and also this GO :0019901: protein kinase binding and also this GO :0030983: mismatched DNA binding and also this GO :0032137: guanine/thymine mispair binding and also this GO :0032139: dinucleotide insertion or deletion binding and also this GO :0032142: single guanine insertion binding and also this GO :0032143: single thymine insertion binding and also this GO :0032181: dinucleotide repeat insertion binding and also this GO :0032357: oxidized purine DNA binding and also this GO :0032405: MutLalpha complex binding and also this GO :0042803: protein homodimerization activity and also this GO :0043531: ADP binding, this GO :0000400: four-way junction DNA binding, this GO :0000403: Y-form DNA binding, this GO :0000404: heteroduplex DNA loop binding, this GO :0000406: double-strand/single-strand DNA junction binding, this GO :0003677: DNA binding, this GO :0003684: damaged DNA binding, this GO :0003690: double-stranded DNA binding, this GO :0003697: single-stranded DNA binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0008022: protein C-terminus binding, this GO :0008094: DNA-dependent ATPase activity, this GO :0016887: ATPase activity, this GO :0019237: centromeric DNA binding, this GO :0019899: enzyme binding, this GO :0019901: protein kinase binding, this GO :0030983: mismatched DNA binding, this GO :0032137: guanine/thymine mispair binding, this GO :0032139: dinucleotide insertion or deletion binding, this GO :0032142: single guanine insertion binding, this GO :0032143: single thymine insertion binding, this GO :0032181: dinucleotide repeat insertion binding, this GO :0032357: oxidized purine DNA binding, this GO :0032405: MutLalpha complex binding, this GO :0042803: protein homodimerization activity, this GO :0043531: ADP binding, mutS homolog 2 |
| Identity | 7325 |
| Gene | MSH2 |
| Long gene name | mutS homolog 2 |
| Synonyms gene | COCA1 |
| Synonyms gene name | coli) , coli) homolog 2 (colon cancer, colon cancer, mutS (E, nonpolyposis type 1 (E, nonpolyposis type 1) mutS homolog 2 |
| Synonyms | HNPCC HNPCC1 |
| Locus | 2p21-p16, 3 |
| Discovery year | 1993-07-28 |
| GenBank acession | U03911 |
| Entrez gene record | 4436 |
| Pubmed identfication | 8484120 9843200 |
| Classification | MutS homologs |
| Havana BLAST/BLAT | OTTHUMG00000128861 |
| Locus Specific Databases | HNPCC Colon cancer gene variant databases MSH2 database at LOVD-China LOVD - Leiden Open Variation Database LRG_218 , Hereditary Non-Polyposis Colorectal Cancer |
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