| Long name | MSH6 Primary Polyclonal Antibody |
| Also known as | MSH6 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | MSH6 |
| Specificity | This is a highly specific antibody against MSH6 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 830-880/1360 |
| Subcellular location | Nucleus |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MSH6 |
| Gene ID number | 2956 |
| Swiss Prot | P52701 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | ATP binding and hydrolysis play a pivotal role in mismatch repair functions, After mismatch binding, Heterodimerizes with MSH2 to form MutS alpha, MutS alpha bends the DNA helix and shields approximately 20 base pairs, MutS alpha may also play a role in DNA homologous recombination repair, Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction, The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->, This transition is crucial for mismatch repair, When bound, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA, and resynthesis, excision, forms a ternary complex with the MutL alpha heterodimer, including strand discrimination, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone, which binds to DNA mismatches thereby initiating DNA repair, which is thought to be responsible for directing the downstream MMR events, ATP exchange, Component of the post-replicative DNA mismatch repair system (MMR) |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTMBP, HNPCC5, HSAP, MSH6, MutS-alpha 160 kDa subunit, hMSH6, p160, GTBP |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | MSH6 |
| Short name | MSH6 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | coli) (Antibody to), mutS homolog 6 (E |
| Alternative technique | antibodies |
| Alternative to gene target | ADP binding, BT, GTBP and GTMBP and HNPCC5 and HSAP and p160, MSH6 and IDBG-51044 and ENSG00000116062 and 2956, Msh6 and IDBG-201548 and ENSMUSG00000005370 and 17688, coli), nuclei, this GO :0000228 and nuclear chromosome and cellular component this GO :0000287 and magnesium ion binding and molecular function this GO :0000400 and four-way junction DNA binding and molecular function this GO :0000710 and meiotic mismatch repair and biological process this GO :0000790 and nuclear chromatin and cellular component this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003684 and damaged DNA binding and molecular function this GO :0003690 and double-stranded DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006298 and mismatch repair and biological process this GO :0007131 and reciprocal meiotic recombination and biological process this GO :0008094 and DNA-dependent ATPase activity and molecular function this GO :0008340 and determination of adult lifespan and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0009411 and response to UV and biological process this GO :0016446 and somatic hypermutation of immunoglobulin genes and biological process this GO :0016447 and somatic recombination of immunoglobulin gene segments and biological process this GO :0016887 and ATPase activity and molecular function this GO :0030983 and mismatched DNA binding and molecular function this GO :0032137 and guanine/thymine mispair binding and molecular function this GO :0032142 and single guanine insertion binding and molecular function this GO :0032143 and single thymine insertion binding and molecular function this GO :0032301 and MutSalpha complex and cellular component this GO :0032357 and oxidized purine DNA binding and molecular function this GO :0032405 and MutLalpha complex binding and molecular function this GO :0035064 and methylated histone binding and molecular function this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0043531 and ADP binding and molecular function this GO :0045190 and isotype switching and biological process this GO :0045910 and negative regulation of DNA recombination and biological process this GO :0051096 and positive regulation of helicase activity and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0000400: four-way junction DNA binding and also this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0003684: damaged DNA binding and also this GO :0003690: double-stranded DNA binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0008094: DNA-dependent ATPase activity and also this GO :0016887: ATPase activity and also this GO :0030983: mismatched DNA binding and also this GO :0032137: guanine/thymine mispair binding and also this GO :0032142: single guanine insertion binding and also this GO :0032143: single thymine insertion binding and also this GO :0032357: oxidized purine DNA binding and also this GO :0032405: MutLalpha complex binding and also this GO :0035064: methylated histone binding and also this GO :0043531: ADP binding, this GO :0000400: four-way junction DNA binding, this GO :0003677: DNA binding, this GO :0003682: chromatin binding, this GO :0003684: damaged DNA binding, this GO :0003690: double-stranded DNA binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0008094: DNA-dependent ATPase activity, this GO :0016887: ATPase activity, this GO :0030983: mismatched DNA binding, this GO :0032137: guanine/thymine mispair binding, this GO :0032142: single guanine insertion binding, this GO :0032143: single thymine insertion binding, this GO :0032357: oxidized purine DNA binding, this GO :0032405: MutLalpha complex binding, this GO :0035064: methylated histone binding, this GO :0043531: ADP binding, 98842 and IDBG-633641 and ENSBTAG00000001424 and 540526, mutS homolog 6 (E |
| Identity | 7329 |
| Gene | MSH6 |
| Long gene name | mutS homolog 6 |
| Synonyms gene | GTBP |
| Synonyms gene name | coli) , coli) homolog 6 mutS homolog 6 (E, mutS (E |
| Locus | 2p16, 3 |
| Discovery year | 1995-08-29 |
| GenBank acession | U54777 |
| Entrez gene record | 2956 |
| Pubmed identfication | 7604266 |
| RefSeq identity | NM_000179 |
| Classification | MutS homologs PWWP domain containing |
| Havana BLAST/BLAT | OTTHUMG00000129129 |
| Locus Specific Databases | HNPCC Colon cancer gene variant databases MSH6 database at LOVD-China LRG_219 , Hereditary Non-Polyposis Colorectal Cancer |
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