| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | NRP2 |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | WB |
| Recommended dilutions | 5-1ug/ml, Western blot: 0 |
| Notes | Titration of the NRP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use | This NRP2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | O60462 |
| Purity | Antigen affinity |
| Description | Mice with null mutations in genes encoding Sema3F, Moreover, NRP2 may play a role in cardiovascular development, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, Sema3b can act to repulse axons expressing neuropilin-2 and can antagonize the repulsive action of Sema3a on axonal growth cones expressing neuropilin-1, These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of Neuropilin-2 to apical dendrites and of NRP1 to all dendrites of cortical pyramidal neurons, also called Npn2 or VEGF165R2, and also aberrant spine distribution, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency, and its holoreceptor components NRP2 and Plexin A3 (PLEXA3) exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and tumorigenesis, axon guidance, is a member of the neuropilin family of receptor proteins, Neuropilin-2 |
| Immunogen | Amino acids 45-60 (YPQDYPSHQNCEWIVY-human) were used as the immunogen for this NRP2 antibody (100% mouse homology) |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the NRP2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | NRP2 |
| Short name | NRP2 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | neuropilin 2 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | Extracellular, NP2 and NPN2 and PRO2714 and VEGF165R2, NRP2 and IDBG-643390 and ENSBTAG00000011971 and 541004, NRP2 and IDBG-79320 and ENSG00000118257 and 8828, Nrp2 and IDBG-160044 and ENSMUSG00000025969 and 18187, metal ion binding, this GO :0001525 and angiogenesis and biological process this GO :0001755 and neural crest cell migration and biological process this GO :0001764 and neuron migration and biological process this GO :0001938 and positive regulation of endothelial cell proliferation and biological process this GO :0002116 and semaphorin receptor complex and cellular component this GO :0004872 and receptor activity and molecular function this GO :0005021 and vascular endothelial growth factor-activated receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007411 and axon guidance and biological process this GO :0007507 and heart development and biological process this GO :0008201 and heparin binding and molecular function this GO :0010595 and positive regulation of endothelial cell migration and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0017154 and semaphorin receptor activity and molecular function this GO :0019838 and growth factor binding and molecular function this GO :0019955 and cytokine binding and molecular function this GO :0021675 and nerve development and biological process this GO :0021828 and this GO nadotrophin-releasing hormone neuronal migration to the hypothalamus and biological process this GO :0030424 and axon and cellular component this GO :0038084 and vascular endothelial growth factor signaling pathway and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048010 and vascular endothelial growth factor receptor signaling pathway and biological process this GO :0048846 and axon extension involved in axon guidance and biological process this GO :0050919 and negative chemotaxis and biological process this GO :0061549 and sympathetic ganglion development and biological process this GO :0097490 and sympathetic neuron projection extension and biological process this GO :0097491 and sympathetic neuron projection guidance and biological process this GO :1902285 and semaphorin-plexin signaling pathway involved in neuron projection guidance and biological process, this GO :0004872: receptor activity, this GO :0004872: receptor activity and also this GO :0005021: vascular endothelial growth factor-activated receptor activity and also this GO :0005515: protein binding and also this GO :0008201: heparin binding and also this GO :0017154: semaphorin receptor activity and also this GO :0019838: growth factor binding and also this GO :0019955: cytokine binding and also this GO :0046872: metal ion binding, this GO :0005021: vascular endothelial growth factor-activated receptor activity, this GO :0005515: protein binding, this GO :0008201: heparin binding, this GO :0017154: semaphorin receptor activity, this GO :0019838: growth factor binding, this GO :0019955: cytokine binding, this GO :0046872: metal ion binding, neuropilin 2 |
| Identity | 7751 |
| Gene | NELL2 |
| Long gene name | neural EGFL like 2 |
| Synonyms gene name | nel (chicken)-like 2 NEL-like 2 (chicken) |
| Synonyms | NRP2 |
| Locus | 12q12 |
| Discovery year | 1996-07-17 |
| GenBank acession | D83018 |
| Entrez gene record | 4753 |
| Pubmed identfication | 8975702 25537860 |
| RefSeq identity | NM_006159 |
| Havana BLAST/BLAT | OTTHUMG00000169464 |
Technique
Supplier
Species
Label
Iso Type
Host
Tissue