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Nucleolin Antibody

Nucleolin Antibody size: 0.08 ml 199

Supplier NJS POLY
Price 199
Size 0.08 ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration pH 7, with 0, 09% sodium azide, 4, In 1X PBS
FormAntigen affinity purified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenNucleolin
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applications ELISA, IF, IHC, WB
Recommended dilutionsIHC (Paraffin): 1:50-1:100, Immunofluorescence: 1:10-1:50, Western blot: 1:1000
Notes Due to differences in protocols and secondary/substrate sensitivity, it may be necessary to titrate the Nucleolin antibody for optimal performance, The stated application concentrations are suggested starting amounts
Intented useThis Nucleolin antibodyis to be used only for research purposes and not for diagnostics
Uniprot #P19338
PurityAntigen affinity
Description Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb, It is located mainly in dense fibrillar regions of the nucleolus, The intron 11 of the NCL gene encodes a small nucleolar RNA, [Wiki], a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes, termed U20, Nucleolin (NCL)
ImmunogenThe amino acid sequence used as immunogen for this Nucleolin antibody is found between 425-455 of the human protein
Storage Avoid repeat freeze-thaws, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, The Nucleolin antibody should be aliquoted and and stored frozen at -20 deg
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetNucleolin
Short nameNucleolin Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameNucleolin (Antibody to)
Alternative techniqueantibodies
Identity 7667
Gene NCL
Long gene name nucleolin
Synonyms C23
Locus 2q37, 1
Discovery year 1991-01-15
Entrez gene record 4691
Pubmed identfication 2394707 3409881
RefSeq identity NM_005381
Classification RNA binding motif containing DNA helicases
Havana BLAST/BLAT OTTHUMG00000153866

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