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PDHA1(Ser293) Antibody, Biotin Conjugated

PDHA1(Ser293) Antibody, Biotin Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name Biotin Conjugated, PDHA1(Ser293) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPDHA1 Ser293
Specificity Ser296 in Mouse, This antibody will detect PDHA2, This phosphorylation site is homologous across the listed species, and the predicted site of Ser296 in Rat due to sequence homology, the testis specific form, when phosphorylated at Ser293 in Human, This is a highly specific antibody against PDHA1 Ser293
ModificationPhosphorylation
Modification siteSer293
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range270-320/390
Subcellular locationMitochondrion
SourceKLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293
Gene ID number5160
Swiss ProtP08559
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background and thereby links the glycolytic pathway to the tricarboxylic cycle, The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPDHA1(Ser293) Conjugated
Short name Conjugated, PDHA1(Ser293) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, PDHA1(Ser293) (Antibody to)
Alternative techniqueantibodies
Identity 8806
Gene PDHA1
Long gene name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms gene PDHA
Synonyms name mitochondrial , pyruvate dehydrogenase E1 component subunit alpha, somatic form
Locus Xp22, 12
Discovery year 1989-06-30
Entrez gene record 5160
Havana BLAST/BLAT OTTHUMG00000021224
Locus Specific Databases Mental Retardation database

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