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PRKR Antibody

PRKR Antibody size: 0.4 ml 406

Supplier NJS POLY
Price 406
Size 0.4 ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration pH 7, with 0, 09% sodium azide, 4, In 1X PBS
FormPurified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenPRKR
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applications ELISA, IHC, WB
Recommended dilutionsIHC (Paraffin): 1:50-1:100, Western blot: 1:1000
Notes Titration of the PRKR antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts
Intented useThis PRKR antibodyis to be used only for research purposes and not for diagnostics
Uniprot #P19525
PurityPurified
Description Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, E2 inhibits the kinase activity of PRKR and blocks its inhibitory effect on protein synthesis and cell growth, Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation, IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region, The HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PRKR and the translation initiation factor EIF2-alpha, a target of PRKR, double-stranded RNA-activated protein kinase is a serine-threonine kinase, leading to the inhibition of protein synthesis, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), which provides one mechanism by which HCV may circumvent the antiviral effect of interferon, Interferon-induced
ImmunogenA portion of amino acids 519-550 from the human protein was used as the immunogen for this PRKR antibody
Storage Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the PRKR antibody and store frozen at -20 deg
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetPRKR
Short namePRKR Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative namePRKR (Antibody to)
Alternative techniqueantibodies
Identity 9437
Gene EIF2AK2
Long gene name eukaryotic translation initiation factor 2 alpha kinase 2
Synonyms gene PRKR
Synonyms gene name interferon-inducible double stranded RNA dependent , protein kinase
Synonyms PKR EIF2AK1 PPP1R83
Synonyms name protein phosphatase 1, regulatory subunit 83
Locus 2p22, 2
Discovery year 1992-12-04
GenBank acession BC057805
Entrez gene record 5610
Pubmed identfication 1351683
RefSeq identity NM_002759
Classification Protein phosphatase 1 regulatory subunits
Havana BLAST/BLAT OTTHUMG00000100962

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