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Recombinant Human NEDD8-Conjugating Enzyme UBC12, UBE2M

Recombinant Human NEDD8-Conjugating Enzyme UBC12, UBE2M size: 1 mg 1014

Supplier NOVO
Price 1014
Size 1 mg
Reacts withHuman
Source proteins, Recombinants or rec
Description Activating enzymes will cut off the domain that is biological active to become functional, If the substrate is DNA they are called restriction enzymes, Enzymes are cleaving the substrate
Molecular Weight20, 9 kD
UniProt numberP61081
State of the productLiquid
Shipping conditionsDry ice/ice packs
Formulation10%glycerol , 150 mM sodium chloride, 2 mM DTT, 2 um filtered solution of 50 mM HEPES, 5, Supplied as a 0, pH 7
Storage recommendations -20°, Please minimize freeze-thaw cycles, stable for 6 months after receipt, C, Store at <
Reconstitution Dissolve the lyophilized protein in ddH2O, Do not mix by vortex or pipetting, It is not recommended to reconstitute to a concentration less than 100 &mu, Please aliquot the reconstituted solution to minimize freeze-thaw cycles, Always centrifuge tubes before opening, g/ml
Purity and determined by Polyacrylamide gel electrophoresis, Greater than 95%
Sequence of the amino acidMIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK
Levels of endotoxin11 IEU/ug, LAL test shows less than than 0
Properties Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, Homo sapiens sapiens), Human proteins, humans , ssp
Grouprecombinants
Gene target UBE2M, NEDD8-Conjugating UBC12
Short name UBE2M, Recombinant NEDD8-Conjugating Enzyme UBC12
Technique E, coli recombinant proteins are , enzymes, novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture, supplied as white sterile powder lyopillized, Recombinant, genetic recombinations , in Escherichia coli
Species Humans, Human
Alternative name UBE2M, sapiens NEDD8-Conjugating Enzyme UBC12, recombinant H
Alternative techniquerec
Identity 12491
Gene UBE2M
Long gene name ubiquitin conjugating enzyme E2 M
Synonyms gene name ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12) ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) ubiquitin-conjugating enzyme E2M
Synonyms hUbc12 UBC12
Locus 19q13, 43
Discovery year 1999-01-22
GenBank acession AB012191
Entrez gene record 9040
Pubmed identfication 9694792
RefSeq identity NM_003969
Classification Ubiquitin conjugating enzymes E2
Havana BLAST/BLAT OTTHUMG00000183548

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