| Long name | RPS6KB1 Primary Polyclonal Antibody |
| Also known as | RPS6KB1 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | RPS6KB1 |
| Specificity | This antibody may detect p70 ribosomal S6 kinase beta 2, This is a highly specific antibody against RPS6KB1 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 300-350/525 |
| Subcellular location | Nucleus, Cytoplasm |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human of human p70 S6 Kinase |
| Gene ID number | 6198 |
| Swiss Prot | P23443 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Also controls translation initiation by phosphorylating a negative regulator of EIF4A, Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, In response to IGF1, May be involved in cytoskeletal rearrangement through binding to neurabin, Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function, PDCD4, Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex, Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR, RPS6 and EEF2K, Regulates protein synthesis through phosphorylation of EIF4B, The active form then phosphorylates and activates several substrates in the pre-initiation complex, The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, Under conditions of nutrient depletion, Upon mitogenic stimulation, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), and contributes to cell survival by repressing the pro-apoptotic function of BAD, cell growth and cell cycle progression, including the EIF2B complex and the cap-binding complex component EIF4B, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation, resulting in accelerated degradation of IRS1, resulting in the inhibition of mTORC2 and AKT1 signaling, targeting it for ubiquitination and subsequent proteolysis, the inactive form associates with the EIF3 translation initiation complex, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function, which leads to its inhibition and thus activation of EEF2, Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | 70 kDa ribosomal protein S6 kinase 1, P70S6K1, PS6K, RPS6KB1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, STK14A, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha, p70-S6K, p70-S6K 1, p70-alpha, S6K |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | RPS6KB1 |
| Short name | RPS6KB1 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | 70kDa, polypeptide 1 (Antibody to), ribosomal protein S6 phosphorylation catalyst |
| Alternative technique | antibodies |
| Alternative to gene target | 70kDa, RPS6KB1 and IDBG-61938 and ENSG00000108443 and 6198, RPS6KB1 and IDBG-629797 and ENSBTAG00000016851 and 404181, Rps6kb1 and IDBG-206895 and ENSMUSG00000020516 and 72508, nuclei, p70 S6KA and p70-alpha and p70-S6K and p70(S6K)-alpha and PS6K and S6K and S6K-beta-1 and S6K1 and STK14A, polypeptide 1, this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0003009 and skeletal muscle contraction and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004711 and ribosomal protein S6 kinase activity and molecular function this GO :0004712 and protein serine/threonine/tyrosine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005741 and mitochondrial outer membrane and cellular component this GO :0005829 and cytosol and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0007165 and signal transduction and biological process this GO :0007281 and germ cell development and biological process this GO :0007568 and aging and biological process this GO :0007584 and response to nutrient and biological process this GO :0007616 and long-term memory and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0009408 and response to heat and biological process this GO :0009611 and response to wounding and biological process this GO :0009612 and response to mechanical stimulus and biological process this GO :0009636 and response to toxic substance and biological process this GO :0009749 and response to glucose and biological process this GO :0009986 and cell surface and cellular component this GO :0010033 and response to organic substance and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0014732 and skeletal muscle atrophy and biological process this GO :0014878 and response to electrical stimulus involved in regulation of muscle adaptation and biological process this GO :0014911 and positive regulation of smooth muscle cell migration and biological process this GO :0016477 and cell migration and biological process this GO :0016772 and transferase activity, this GO :0004672: protein kinase activity, this GO :0004672: protein kinase activity and also this GO :0004674: protein serine/threonine kinase activity and also this GO :0004711: ribosomal protein S6 kinase activity and also this GO :0004712: protein serine/threonine/tyrosine kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004674: protein serine/threonine kinase activity, this GO :0004711: ribosomal protein S6 kinase activity, this GO :0004712: protein serine/threonine/tyrosine kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, this GO :0042277: peptide binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0042277: peptide binding, transferring phosphorus-containing groups and molecular function this GO :0030054 and cell junction and cellular component this GO :0031929 and TOR signaling and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032868 and response to insulin and biological process this GO :0032870 and cellular response to hormone stimulus and biological process this GO :0033574 and response to testosterone and biological process this GO :0033762 and response to gluca this GO n and biological process this GO :0034612 and response to tumor necrosis factor and biological process this GO :0042277 and peptide binding and molecular function this GO :0042493 and response to drug and biological process this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043200 and response to amino acid and biological process this GO :0043201 and response to leucine and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0043491 and protein kinase B signaling and biological process this GO :0045202 and synapse and cellular component this GO :0045471 and response to ethanol and biological process this GO :0045727 and positive regulation of translation and biological process this GO :0045931 and positive regulation of mitotic cell cycle and biological process this GO :0045948 and positive regulation of translational initiation and biological process this GO :0046324 and regulation of glucose import and biological process this GO :0046627 and negative regulation of insulin receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048633 and positive regulation of skeletal muscle tissue growth and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process, ribosomal protein S6 kinase |
| Identity | 10436 |
| Gene | RPS6KB1 |
| Long gene name | ribosomal protein S6 kinase B1 |
| Synonyms gene | STK14A |
| Synonyms gene name | 70kD, polypeptide 1 , ribosomal protein S6 kinase |
| Synonyms | S6K1 p70(S6K)-alpha PS6K |
| Locus | 17q23, 1 |
| Discovery year | 1994-07-11 |
| GenBank acession | M60724 |
| Entrez gene record | 6198 |
| Pubmed identfication | 1922062 |
| RefSeq identity | NM_003161 |
| Havana BLAST/BLAT | OTTHUMG00000150642 |
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