| Long name | RUNX1 (Ser249) Primary Polyclonal Antibody |
| Also known as | RUNX1 (Ser249) PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | RUNX1 Ser249 |
| Specificity | This is a highly specific antibody against RUNX1 Ser249 |
| Modification(s) | Phosphorylation |
| Modification site(s) | Ser249 |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic phosphopeptide derived from human RUNX1 around the phosphorylation site of Ser249 [QP(p-S)PP] |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | 5'-PYGPYGGT-3', AML1/Runx1 is expressed in a wide variety of tissues and is expressed at the highest levels in thymus, CBF binds to the core site, DNA binding is increased by heterodimerization with CBFB, Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, IL3 and GMCSF promoters, Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1, LCK, T cell receptor enhancers, The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia, bone marrow and peripheral blood, including murine leukemia virus, of a number of enhancers and promoters, polyomavirus enhancer, AML1/Runx1 binds DNA as a monomer and through the Runt domain |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | AML 1, AML1, AML1 EVI 1, AMLCR 1, AMLCR1, Acute myeloid leukemia 1, Acute myeloid leukemia 1 protein, Aml1 oncogene, CBFA 2, CBFA2, Core binding factor alpha 2 subunit, Core binding factor runt domain alpha subunit 2, EVI 1, EVI1, HGNC, Oncogene AML 1, PEA2 alpha, PEBP2 alpha B, PEBP2A2, PEBP2aB, Polyomavirus enhancer binding protein 2 alpha B subunit, RUNX 1, RUNX1 phospho S249, RUNX1 phospho Ser249, RUNX1_HUMAN, Run1, Runt related transcription factor 1, SL3 3 enhancer factor 1 alpha B subunit, SL3/AKV core binding factor alpha B subunit, alpha subunit core binding factor, p-RUNX1 Ser249, RUNX1 Ser249 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | RUNX1 (Ser249) |
| Short name | RUNX1 (Ser249) Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | runt-related transcription factor 1 (Ser249) (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | AML1 and AML1-EVI-1 and AMLCR1 and CBFA2 and EVI-1 and PEBP2aB, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0007417 and central nervous system development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0009966 and regulation of signal transduction and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030099 and myeloid cell differentiation and biological process this GO :0030182 and neuron differentiation and biological process this GO :0030853 and negative regulation of granulocyte differentiation and biological process this GO :0030854 and positive regulation of granulocyte differentiation and biological process this GO :0031069 and hair follicle morphogenesis and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048266 and behavioral response to pain and biological process this GO :0048666 and neuron development and biological process this GO :0048935 and peripheral nervous system neuron development and biological process this GO :0060216 and definitive hemopoiesis and biological process this GO :0070491 and repressing transcription factor binding and molecular function this GO :0071336 and regulation of hair follicle cell proliferation and biological process this GO :0071425 and hematopoietic stem cell proliferation and biological process this GO :0071560 and cellular response to transforming growth factor beta stimulus and biological process this GO :2000872 and positive regulation of progesterone secretion and biological process, RUNX1 and IDBG-2825 and ENSG00000159216 and 100506403, RUNX1 and IDBG-640443 and ENSBTAG00000004742 and 529631, Runx1 and IDBG-177713 and ENSMUSG00000022952 and 12394, nuclei, repressing transcription factor binding, this GO :0000975 and regulatory region DNA binding and molecular function this GO :0001501 and skeletal system development and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001889 and liver development and biological process this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005604 and basement membrane and cellular component this GO :0005634 and nucleus and cellular component this GO :0006351 and transcription, this GO :0000975: regulatory region DNA binding, this GO :0000975: regulatory region DNA binding and also this GO :0003677: DNA binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0005509: calcium ion binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0008134: transcription factor binding and also this GO :0042803: protein homodimerization activity and also this GO :0046982: protein heterodimerization activity and also this GO :0070491: repressing transcription factor binding, this GO :0003677: DNA binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0005509: calcium ion binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0008134: transcription factor binding, this GO :0042803: protein homodimerization activity, this GO :0046982: protein heterodimerization activity, this GO :0070491: repressing transcription factor binding, 861, 101928269, runt-related transcription factor 1 |
| Identity | 10471 |
| Gene | RUNX1 |
| Long gene name | runt related transcription factor 1 |
| Synonyms gene | AML1 CBFA2 |
| Synonyms gene name | acute myeloid leukemia 1 runt-related transcription factor 1 |
| Synonyms | PEBP2A2 AMLCR1 |
| Synonyms name | aml1 oncogene |
| Locus | 21q22, 12 |
| Discovery year | 1991-08-20 |
| GenBank acession | X79549 |
| Entrez gene record | 861 |
| Pubmed identfication | 1427868 7835892 |
| Havana BLAST/BLAT | OTTHUMG00000086299 |
| Locus Specific Databases | LRG_482 |
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