| Long name | SIAH1 Primary Polyclonal Antibody |
| Also known as | SIAH1 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | SIAH1 |
| Specificity | This is a highly specific antibody against SIAH1 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 200-250/282 |
| Subcellular location | Nucleus, Cytoplasm |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SIAH1 |
| Gene ID number | 6477 |
| Swiss Prot | Q8IUQ4 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Confers constitutive instability to HIPK2 through proteasomal degradation, E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, GAPDH acts as a stabilizer of SIAH1, Has some overlapping function with SIAH2, Induces apoptosis in cooperation with PEG3, It is thereby involved in many cellular processes such as apoptosis, MYB, Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes, PML and RBBP8), POU2AF1, Triggers the ubiquitin-mediated degradation of many substrates, Upon nitric oxid (NO) generation that follows apoptotic stimulation, a cell surface receptor (DCC), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP, an antiapoptotic protein (BAG1), axon guidance, cell cycle, facilitating the degradation of nuclear proteins, including proteins involved in transcription regulation (ELL2, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus, spermatogenesis and TNF-alpha signaling, the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), transcription regulation, tumor suppression, E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | E3 ubiquitin-protein ligase SIAH1, HUMSIAH, SIAH1, Seven in absentia homolog 1, Siah-1, Siah-1a, SIAH1A |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | SIAH1 |
| Short name | SIAH1 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | siah E3 ubiquitin protein ligase 1 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | BT, SIAH1 and IDBG-30112 and ENSG00000196470 and 6477, SIAH1A, ligase activity, nuclei, this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005769 and early endosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006508 and proteolysis and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0007049 and cell cycle and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007399 and nervous system development and biological process this GO :0007411 and axon guidance and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0016874 and ligase activity and molecular function this GO :0030163 and protein catabolic process and biological process this GO :0030877 and beta-catenin destruction complex and cellular component this GO :0031648 and protein destabilization and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0051402 and neuron apoptotic process and biological process this GO :2001244 and positive regulation of intrinsic apoptotic signaling pathway and biological process, this GO :0004842: ubiquitin-protein transferase activity, this GO :0004842: ubiquitin-protein transferase activity and also this GO :0005515: protein binding and also this GO :0008022: protein C-terminus binding and also this GO :0008270: zinc ion binding and also this GO :0016874: ligase activity, this GO :0005515: protein binding, this GO :0008022: protein C-terminus binding, this GO :0008270: zinc ion binding, this GO :0016874: ligase activity, 37798 and IDBG-631496 and ENSBTAG00000010868 and 534655, siah E3 ubiquitin protein ligase 1 |
| Identity | 10857 |
| Gene | SIAH1 |
| Long gene name | siah E3 ubiquitin protein ligase 1 |
| Synonyms gene name | seven in absentia homolog 1 (Drosophila) |
| Synonyms | hSIAH1 |
| Locus | 16q12, 1 |
| Discovery year | 1997-09-12 |
| GenBank acession | U76247 |
| Entrez gene record | 6477 |
| Pubmed identfication | 9403064 9334332 |
| Havana BLAST/BLAT | OTTHUMG00000175417 |
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