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SKAP55 Antibody, FITC Conjugated

SKAP55 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, SKAP55 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenSKAP55
SpecificityThis is a highly specific antibody against SKAP55
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human SKAP55
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background SKAP55 and SKAP55-R bind to Fyb through their SH3 domains and function as substrates for p59Fyn in resting T cells, SKAP55 associates with SLAP-130, SKAP55 contains an N-terminal pleckstrin homology domain and a C-terminal SH3 domain binding motif of adjacent arginine and lysine residues followed by tandem tyrosines, SKAP55-R, The human SKAP55 gene maps to chromosome 17q21, another component of the Fyn complex, contains a coiled-coil N-terminal domain, similar in overall structure to SKAP55, which plays a role in the regulation of signaling events initiated by lymphocyte antigen receptors leading up to T cell activation, 32 and encodes a 359 amino acid protein, Fyb (Fyn binding protein) and the anchoring proteins SKAP55 and SKAP55-R (SKAP55-related protein) associate with the tyrosine kinase p59fyn
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms SKAP55
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetSKAP55 Conjugated
Short name FITC Conjugated, SKAP55 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, SKAP55 (Antibody to)
Alternative techniqueantibodies
Identity 15605
Gene SKAP1
Long gene name src kinase associated phosphoprotein 1
Synonyms gene SCAP1
Synonyms gene name src family associated phosphoprotein 1
Locus 17q21, 32
Discovery year 2001-06-21
GenBank acession Y11215
Entrez gene record 8631
Pubmed identfication 9195899
RefSeq identity NM_003726
Classification Pleckstrin homology domain containing
Havana BLAST/BLAT OTTHUMG00000178823

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