| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | XRCC3 |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | WB |
| Recommended dilutions | 5-1ug/ml, Western blot: 0 |
| Notes | Titration of the XRCC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use | This XRCC3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | O43542 |
| Purity | Antigen affinity |
| Description | A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity, Its function is not limited to HR initiation, The gene functionally complements Chinese hamster irs1SF, The protein is a member of the RecA/Rad51-related family that participates in homologous recombination to maintain chromosome stability and repair DNA damage, XRCC3 interacts directly with RAD51 and may cooperate with RAD51 during recombinational repair, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable, but extends to later stages in formation and resolution of HR intermediates, complementing defective, in chinese hamster 3 is a DNA repair protein that in humans, is encoded by the XRCC3 gene, possibly by stabilizing heteroduplex DNA, X-ray repair |
| Immunogen | An amino acid sequence from the middle region of human XRCC3 (HKRLQQLMAQQPRLRTD) was used as the immunogen for this XRCC3 antibody |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the XRCC3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | XRCC3 |
| Short name | XRCC3 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | X-ray repair complementing defective repair in Chinese hamster cells 3 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | CMM6, XRCC3 and IDBG-22058 and ENSG00000126215 and 7517, XRCC3 and IDBG-635474 and ENSBTAG00000009966 and 768244, Xrcc3 and IDBG-173748 and ENSMUSG00000021287 and 74335, crossover junction endodeoxyribonuclease activity, endonucleolytic and biological process this GO :0003677 and DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005657 and replication fork and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006259 and DNA metabolic process and biological process this GO :0006281 and DNA repair and biological process this GO :0006310 and DNA recombination and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0008094 and DNA-dependent ATPase activity and molecular function this GO :0008821 and crossover junction endodeoxyribonuclease activity and molecular function this GO :0010033 and response to organic substance and biological process this GO :0010824 and regulation of centrosome duplication and biological process this GO :0033065 and Rad51C-XRCC3 complex and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0071140 and resolution of mitotic recombination intermediates and biological process this GO :0090267 and positive regulation of mitotic cell cycle spindle assembly checkpoint and biological process, nuclei, this GO :0000400 and four-way junction DNA binding and molecular function this GO :0000724 and double-strand break repair via homolo this GO us recombination and biological process this GO :0000737 and DNA catabolic process, this GO :0000400: four-way junction DNA binding, this GO :0000400: four-way junction DNA binding and also this GO :0003677: DNA binding and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0008094: DNA-dependent ATPase activity and also this GO :0008821: crossover junction endodeoxyribonuclease activity, this GO :0003677: DNA binding, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0008094: DNA-dependent ATPase activity, this GO :0008821: crossover junction endodeoxyribonuclease activity, X-ray repair complementing defective repair in Chinese hamster cells 3 |
| Identity | 12830 |
| Gene | XRCC3 |
| Long gene name | X-ray repair cross complementing 3 |
| Synonyms gene name | X-ray repair complementing defective repair in Chinese hamster cells 3 |
| Synonyms name | RAD51-like |
| Locus | 14q32, 3 |
| Discovery year | 1995-08-11 |
| GenBank acession | AF035586 |
| Entrez gene record | 7517 |
| Pubmed identfication | 7603995 |
| RefSeq identity | NM_005432 |
| Havana BLAST/BLAT | OTTHUMG00000169228 |
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