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14-3-3 protein zeta/delta (S58) Antibody, Biotin Conjugated

14-3-3 protein zeta/delta (S58) Antibody, Biotin Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name Biotin Conjugated, 14-3-3 protein zeta/delta (S58) Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target Antigen14-3-3 protein zeta/delta Ser58
Specificity This antibody will react with all 14-3-3 proteins containing the conserved motif RS(S-p)WR, This is a highly specific antibody against 14-3-3 protein zeta/delta Ser58
ModificationPhosphorylation
Modification siteSer58
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range30-70/245
Subcellular locationCytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58 [RS(p-S)WR]
Gene ID number 7529, 7534, 10971
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases, Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions, They are also present, and are axonally transported to the nerve terminals, at lower levels, in various other eukaryotic tissues, including those involved in cell cycle regulation and cell survival, localized in neurons, Members of the 14-3-3 family of proteins are highly conserved proteins
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms HS1 14-3-3
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene target14-3-3 protein zeta/delta (S58) Conjugated
Short name Conjugated, 14-3-3 protein zeta/delta (S58) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, 14-3-3 protein zeta/delta (S58) (Antibody to)
Alternative techniqueantibodies
Identity 12854
Gene YWHAQ
Long gene name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
Synonyms gene name theta , theta polypeptide protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein
Synonyms name protein tau
Locus 2p25, 1
Discovery year 1999-08-20
GenBank acession AF070556
Entrez gene record 10971
Pubmed identfication 11080204
RefSeq identity NM_006826
Classification 14-3-3 phospho-serine/phospho-threonine binding proteins
Havana BLAST/BLAT OTTHUMG00000013848

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