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ERK1(T202/Y204)+ERK2(T183/Y185) Antibody, FITC Conjugated

ERK1(T202/Y204)+ERK2(T183/Y185) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, ERK1(T202/Y204)+ERK2(T183/Y185) Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenERK1 Thr202/Tyr204+ERK2 Thr183/Tyr185
SpecificityThis is a highly specific antibody against ERK1 Thr202/Tyr204+ERK2 Thr183/Tyr185
ModificationPhosphorylation
Modification siteThr183/Tyr185
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range155-205/360
Subcellular locationCytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human ERK2 around the phosphorylation site of Thr183/Tyr185
Gene ID number55945595
Swiss ProtP28482
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background MAP kinases, The activation of this kinase requires its phosphorylation by upstream kinases, Two alternatively spliced transcript variants encoding the same protein, Upon activation, act as an integration point for multiple biochemical signals, also known as extracellular signal-regulated kinases (ERKs), and are involved in a wide variety of cellular processes such as proliferation, but differing in the UTRs, differentiation, have been reported for this gene, this kinase translocates to the nucleus of the stimulated cells, transcription regulation and development, where it phosphorylates nuclear targets, The protein encoded by this gene is a member of the MAP kinase family
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms ERK ERK2 p41mapk MAPK2
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetERK1(T202/Y204)+ERK2(T183/Y185) Conjugated
Short name FITC Conjugated, ERK1(T202/Y204)+ERK2(T183/Y185) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ERK1(T202/Y204)+ERK2(T183/Y185) (Antibody to)
Alternative techniqueantibodies
Identity 6871
Gene MAPK1
Long gene name mitogen-activated protein kinase 1
Synonyms gene PRKM2 PRKM1
Locus 22q11, 22
Discovery year 1993-11-05
GenBank acession M84489
Entrez gene record 5594
Classification Mitogen-activated protein kinases
Havana BLAST/BLAT OTTHUMG00000030508
Locus Specific Databases LRG_786

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