RNF74 Antibody

RNF74 Antibody size: 0.1ml 263

Supplier BIOSS PRIMARY UNCONJUGATED ANTIBODIES

Price 263

Size 0.1ml

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Long name	RNF74 Primary Polyclonal Antibody
Also known as	RNF74 PAb
Category	Primary Antibodies
Conjugation	Unconjugated
Target Antigen	RNF74
Specificity	This is a highly specific antibody against RNF74
Modification(s)	None
Modification site(s)	Unmodified antibody
Clonality	Polyclonal antibody
Clone number	Polyclonal antibody
Concentration	1ug per 1ul
Immunogen range	385-430/1043
Subcellular location	Nucleus
Source	This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human RAG1/RNF74
Gene ID number	5896
Swiss Prot	P15918
Tested Applications	IF(IHC-P), IHC-P, WB
Recommended dilutions	IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000)
Cross reactivity	Mouse, Rat, Human
Cross reactive species details	not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the target antigen	5'-phosphorylated ends, DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, Histone H3 monoubiquitination is required for the joining step of V(D)J recombination, In addition to its endonuclease activity, In the RAG complex, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment, RAG2 is not a catalytic component but is required for all known catalytic activities, The RAG complex also plays a role in pre-B cell allelic exclusion, The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps, The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes, and J (joining) gene segments, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, in some cases D (diversity), preventing accessibility to the RAG complex and recombination of the second allele, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, Catalytic component of the RAG complex
Purification method	This antibody was purified via Protein A
Storage conditions	50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA
Synonym names	RAG1, RING finger protein 74, RNF74, V(D)J recombination-activating protein 1, RAG-1
Properties	C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°
Gene target	RNF74
Short name	RNF74 Antibody
Technique	antibodies against human proteins, antibodies for, Antibody
Alternative name	RNF74 (Antibody to)
Alternative technique	antibodies
Identity	9831
Gene	RAG1
Long gene name	recombination activating 1
Synonyms gene name	recombination activating gene 1
Synonyms	RNF74 MGC43321
Synonyms name	recombination activating protein 1 RING finger protein 74 V(D)J recombination-activating protein 1
Locus	11p12
Discovery year	1990-06-18
GenBank acession	M29474
Entrez gene record	5896
Pubmed identfication	1612612 1283330
RefSeq identity	NM_000448
Classification	Ring finger proteins
Havana BLAST/BLAT	OTTHUMG00000166392
Locus Specific Databases	RAG1base: Mutation registry for autosomal recessive RAG1 deficiency LRG_98

RNF74 Antibody

RNF74 Antibody size: 0.1ml 263

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