| Long name | KAP1 Primary Polyclonal Antibody |
| Also known as | KAP1 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | KAP1 |
| Specificity | This is a highly specific antibody against KAP1 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 650-700/835 |
| Subcellular location | Nucleus |
| Source | This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human KAP1/TIF1b |
| Gene ID number | 10155 |
| Swiss Prot | Q13263 |
| Tested Applications | IF(IHC-P), IHC-P, WB |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity | Mouse, Rat, Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Also corepressor for ERBB4, Also specifically sumoylates IRF7, Enhances transcriptional repression by coordinating the increase in H3K9me, Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger, Important regulator of CDKN1A/p21(CIP1), Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation, May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1, May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1, Mediates gene silencing by recruiting CHD3, Mediates the nuclear localization of KOX1, Recruitment of SETDB1 induces heterochromatinization, Ubiquitinates p53/TP53 leading to its proteosomal degradation, ZNF268 and ZNF300 transcription factors, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes, and possibly MAGEA3 and MAGEA6, respectively) and the disposition of HP1 proteins to silence gene expression, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, the function is enhanced by MAGEC2 and MAGEA2, thereby inhibiting its transactivation activity, Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs) |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | E3 SUMO-protein ligase TRIM28, KAP-1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, PPP1R157, RING finger protein 96, RNF96, TF1B, TIF1-beta, TIF1B, TRIM28, Transcription intermediary factor 1-beta, Tripartite motif-containing protein 28, KAP1 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | KAP1 |
| Short name | KAP1 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | KAP1 (Antibody to) |
| Alternative technique | antibodies |
| Identity | 16384 |
| Gene | TRIM28 |
| Long gene name | tripartite motif containing 28 |
| Synonyms gene name | tripartite motif-containing 28 |
| Synonyms | TIF1B KAP1 TF1B RNF96 PPP1R157 |
| Synonyms name | protein phosphatase 1, regulatory subunit 157 |
| Locus | 19q13, 4 |
| Discovery year | 2001-08-10 |
| Entrez gene record | 10155 |
| Pubmed identfication | 11331580 11226167 |
| RefSeq identity | NM_005762 |
| Classification | Tripartite motif containing PHD finger proteins Ring finger proteins Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT | OTTHUMG00000183546 |
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