| Long name | S100A8 Primary Polyclonal Antibody |
| Also known as | S100A8 PAb |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | S100A8 |
| Specificity | This is a highly specific antibody against S100A8 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Polyclonal antibody |
| Clone number | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen range | 20-60/89 |
| Subcellular location | Cell membrane, Secreted, Cytoplasm |
| Source | KLH conjugated synthetic peptide derived from mouse S100-A8 |
| Gene ID number | 6279 |
| Swiss Prot | P05109 |
| Tested Applications | IF(IHC-P), IHC-P |
| Recommended dilutions | IF(IHC-P)(1:50-200), IHC-P(1:100-500) |
| Cross reactivity | Rat, Mouse |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER), Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade, Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3, Can regulate neutrophil number and apoptosis by an anti-apoptotic effect, Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth, It can induce neutrophil chemotaxis and adhesion, Its proinflammatory activity includes recruitment of leukocytes, Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions, The extracellular functions involve proinfammatory, The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, an essential cofactor, and regulation of leukocyte adhesion and migration, antimicrobial, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase, oxidant-scavenging and apoptosis-inducing activities, promotion of cytokine and chemokine production, regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX, transferring arachidonic acid, S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | 60B8AG, CAGA, CFAG, CGLA, CP-10, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, L1Ag, Leukocyte L1 complex light chain, MA387, MIF, MRP-8, MRP8, Migration inhibitory factor-related protein 8, NIF, Protein S100-A8, S100 calcium-binding protein A8, S100A8, Urinary stone protein band A, P8 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target | S100A8 |
| Short name | S100A8 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | S100 calcium binding protein A8 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | 60B8AG and CAGA and CFAG and CGLA and CP-10 and L1Ag and MA387 and MIF and MRP8 and NIF and P8, RAGE receptor binding, S100A8 and IDBG-102654 and ENSG00000143546 and 6279, S100A8 and IDBG-632868 and ENSBTAG00000012640 and 616818, S100a8 and IDBG-168077 and ENSMUSG00000056054 and 20201, nuclei, this GO :0001816 and cytokine production and biological process this GO :0002523 and leukocyte migration involved in inflammatory response and biological process this GO :0002526 and acute inflammatory response and biological process this GO :0002544 and chronic inflammatory response and biological process this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006914 and autophagy and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0008017 and microtubule binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0010043 and response to zinc ion and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030593 and neutrophil chemotaxis and biological process this GO :0032119 and sequestering of zinc ion and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032602 and chemokine production and biological process this GO :0035662 and Toll-like receptor 4 binding and molecular function this GO :0042060 and wound healing and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0045087 and innate immune response and biological process this GO :0045471 and response to ethanol and biological process this GO :0050544 and arachidonic acid binding and molecular function this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0050786 and RAGE receptor binding and molecular function this GO :0050832 and defense response to fungus and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051493 and regulation of cytoskeleton organization and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070488 and neutrophil aggregation and biological process this GO :2001244 and positive regulation of intrinsic apoptotic signaling pathway and biological process, this GO :0005509: calcium ion binding, this GO :0005509: calcium ion binding and also this GO :0005515: protein binding and also this GO :0008017: microtubule binding and also this GO :0008270: zinc ion binding and also this GO :0035662: Toll-like receptor 4 binding and also this GO :0050544: arachidonic acid binding and also this GO :0050786: RAGE receptor binding, this GO :0005515: protein binding, this GO :0008017: microtubule binding, this GO :0008270: zinc ion binding, this GO :0035662: Toll-like receptor 4 binding, this GO :0050544: arachidonic acid binding, this GO :0050786: RAGE receptor binding, S100 calcium binding protein A8 |
| Identity | 10498 |
| Gene | S100A8 |
| Long gene name | S100 calcium binding protein A8 |
| Synonyms gene | CAGA CFAG |
| Synonyms gene name | S100 calcium-binding protein A8 (calgranulin A) S100 calcium binding protein A8 (calgranulin A) |
| Synonyms | P8 MRP8 60B8AG CGLA |
| Locus | 1q21, 3 |
| Discovery year | 1989-05-19 |
| GenBank acession | BC005928 |
| Entrez gene record | 6279 |
| RefSeq identity | NM_002964 |
| Classification | S100 calcium binding proteins EF-hand domain containing |
| Havana BLAST/BLAT | OTTHUMG00000013124 |
Technique
Supplier
Species
Label
Iso Type
Host
Tissue