| Category | Antibody |
| Concentration | pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | H4 |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | DB, ELISA, FACS, WB, IHC |
| Recommended dilutions | Dot blot: 1:500, Flow Cytometry: 1:10-1:50, IHC (Paraffin): 1:50-1:100, Western blot: 1:1000 |
| Notes | Titration of the H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use | This H4 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | P62805 |
| Purity | Antigen affinity |
| Description | A diverse and elaborate array of post-translational modifications including acetylation, H2A, H4, The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, and ADP-ribosylation occurs on the N-terminal tail domains of histones, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers, methylation, phosphorylation, surrounded by approximately 146 base pairs of DNA, ubiquitination, Histone proteins H3 |
| Immunogen | Amino acids surrounding the K20 methylation site of the human protein were used as the immunogen for this Histone H4 antibody |
| Storage | Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the H4 antibody and store frozen at -20 deg |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | H4 |
| Short name | H4 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | H4 (Antibody to) |
| Alternative technique | antibodies |
| Identity | 20510 |
| Gene | HIST4H4 |
| Long gene name | histone cluster 4 H4 |
| Synonyms gene name | H4 , H4 histone cluster 4, histone 4 |
| Synonyms | MGC24116 |
| Locus | 12p12, 3 |
| Discovery year | 2003-02-20 |
| GenBank acession | AY128653 |
| Entrez gene record | 121504 |
| Pubmed identfication | 12408966 |
| RefSeq identity | NM_175054 |
| Classification | Histones |
| Havana BLAST/BLAT | OTTHUMG00000168735 |